Technology type 'Enzymatic Activity Measurements'
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Related assays
8 Assays visible to you, out of a total of 8Intracellular metabolite concentrations were determined enzymatically by measuring the oxidation or reduction of NADH or NAD+, respectively, at 340 nm in a Beckman Coulter DU640 spectrophotometer
Submitter: Katy Wolstencroft
Assay type: Intracellular Metabolite Concentration
Technology type: Enzymatic Activity Measurements
Investigation: Glycerol Synthesis in Saccharomyces cerevisiae
Study: Glycerol Synthesis Pathway
Kinetic characterisation of Phosphoglycerokinase
Submitter: Jacky Snoep
Assay type: Experimental Assay Type
Technology type: Initial Rate Experiment
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Model gluconeogenesis
Submitter: Jacky Snoep
Assay type: Experimental Assay Type
Technology type: Initial Rate Experiment
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Model gluconeogenesis
Submitter: Jacky Snoep
Assay type: Experimental Assay Type
Technology type: Initial Rate Experiment
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Model gluconeogenesis
Kinetic characterization of the FBPA/ase with respect to GAP en DHAP saturation.
Submitter: Jacky Snoep
Assay type: Experimental Assay Type
Technology type: Initial Rate Experiment
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Model gluconeogenesis
GAP and DHAP concentrations were measured over time at 70C.
Submitter: Jacky Snoep
Assay type: Experimental Assay Type
Technology type: Progressive Curve Experiment
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Model gluconeogenesis
The four purified enzymes were incubated in assay buffer and consumption of 3PG and production of F6P were measured in time, together with GAP and DHAP concentrations.
Submitter: Jacky Snoep
Assay type: Experimental Assay Type
Technology type: Progressive Curve Experiment
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Carbon loss at high T
Experimental analysis of pathway fluxes with varying PGK and FBPAase concentrations in the incubation assay.
Submitter: Jacky Snoep
Assay type: Extracellular Metabolite Concentration
Technology type: Progressive Curve Experiment
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Pathway optimisation