P2
Not specified
Not specified
Not specified
Not specified
wild-type
wild-type
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Created: 28th Apr 2010 at 16:17
Last updated: 18th Jul 2012 at 12:26
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Kinetic characterisation of Phosphoglycerokinase
Submitter: Jacky Snoep
Assay type: Experimental Assay Type
Technology type: Initial Rate Experiment
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Model gluconeogenesis
Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)
SOPs: Expression of S. solfataricus enzymes in E. col..., Preparation of cell free extract of recombinant..., Protein purification from E. coli extracts.
Data files: Model simulation and experimental data for ADP,..., PGK kinetics 3PG, PGK kinetics ADP, PGK kinetics ATP, PGK kinetics BPG
Snapshots: No snapshots
Submitter: Jacky Snoep
Assay type: Experimental Assay Type
Technology type: Initial Rate Experiment
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Model gluconeogenesis
Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)
SOPs: Expression of S. solfataricus enzymes in E. col..., Preparation of cell free extract of recombinant..., Protein purification from E. coli extracts.
Data files: GAPDH kinetics 3PG, GAPDH kinetics GAP, GAPDH kinetics NADP, GAPDH kinetics NADPH, GAPDH kinetics Pi
Snapshots: No snapshots
Submitter: Jacky Snoep
Assay type: Experimental Assay Type
Technology type: Initial Rate Experiment
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Model gluconeogenesis
Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)
SOPs: Expression of S. solfataricus enzymes in E. col..., Preparation of cell free extract of recombinant..., Protein purification from E. coli extracts.
Data files: Model simulation and exp data for TIM kinetics,..., TPI kinetics DHAP, TPI kinetics GAP, TPI kinetics PEP inhibition GAP, TPI kinetics PEP inhibition of DHAP
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Kinetic characterization of the FBPA/ase with respect to GAP en DHAP saturation.
Submitter: Jacky Snoep
Assay type: Experimental Assay Type
Technology type: Initial Rate Experiment
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Model gluconeogenesis
Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)
SOPs: Expression of S. solfataricus enzymes in E. col..., Preparation of cell free extract of recombinant..., Protein purification from E. coli extracts.
Data files: FBPA/ase kinetics DHAP, FBPA/ase kinetics GAP, Model simulation and experimental data for DHAP...
Snapshots: No snapshots
Mathematical model for FBPAase kinetics, saturation of GAP and DHAP
Submitter: Jacky Snoep
Biological problem addressed: Model Analysis Type
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Model gluconeogenesis
Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)
Models: FBPAase kinetic model
SOPs: No SOPs
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Snapshots: No snapshots