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SEEK ID: https://demo.fairdomhub.org/studies/9
Central Carbon Metabolism of Sulfolobus solfataricus
Projects: SulfoSys
Experimentalists: Theresa Kouril
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Created: 29th Jan 2013 at 14:11
Last updated: 28th May 2015 at 08:50

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Silicon cell model for the central carbohydrate metabolism of the archaeon Sulfolobus solfataricus under temperature variation
Programme: SysMO
Public web page: http://sulfosys.com/
Organisms: Sulfolobus solfataricus
An investigation in the CCM of S. solfataricus with a focus on the unique temperature adaptations and regulation; using a combined modelling and experimental approach.
Snapshots: Snapshot 1
Studies: Carbon loss at high T, Model gluconeogenesis, Pathway optimisation
Assays: Experimental assays of the temperature dependent degradation of GAP and ..., FBPA/ase, FBPAase model, GAPDH, GAPDH modeling, Gluconeogenic model, Model reconstituted system, Modelling pathway optimisation, Modelling temperature dependent degradation of GAP and DHAP, PGK, PGK and FBPAase titrations, PGK modeling, Reconstituted system reference state, TPI, TPI modeling
Scales: Not specified
The four purified enzymes were incubated in assay buffer and consumption of 3PG and production of F6P were measured in time, together with GAP and DHAP concentrations.
Submitter: Jacky Snoep
Assay type: Experimental Assay Type
Technology type: Progressive Curve Experiment
Snapshots: No snapshots
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Carbon loss at high T
Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)
SOPs: No SOPs
Data files: Metabolite concentrations during reconstituted ..., Model simulation and Exp data for reconstituted...
Scales: Not specified
Mathematical model for the reconstituted system with PGK, GAPDH, TPI and FBPAase.
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Carbon loss at high T
Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)
Models: Mathematical model for the combined four enzyme...
SOPs: No SOPs
Data files: Metabolite concentrations during reconstituted ..., Model simulation and Exp data for reconstituted...
Scales: Not specified
Experimental data for the reconstituted system are plotted together with the model prediction
Creator: Jacky Snoep
Submitter: Jacky Snoep
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Carbon loss at high T
Assays: Model reconstituted system, Reconstituted system reference state
Scales: Not specified
The purified enzymes, PGK, GAPDH, TPI and FBPAase were incubated at 70 C en conversion of 3PG to F6P was followed
Creator: Jacky Snoep
Submitter: Jacky Snoep
Scales: Not specified
The PGK, GAPDH, TPI and FBPAase were modelled together using the individual rate equations. Closed system.
Creator: Jacky Snoep
Submitter: Jacky Snoep
Model type: Ordinary differential equations (ODE)
Model format: SBML
Environment: JWS Online
Organism: Sulfolobus solfataricus
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Carbon loss at high T, Model gluconeogenesis, Pathway optimisation
Modelling analyses: Gluconeogenic model, Model reconstituted system, Modelling pathway optimisation
Scales: Not specified
Abstract (Expand)
Authors: T. Kouril, D. Esser, J. Kort, H. V. Westerhoff, B. Siebers, J. L. Snoep
Date Published: 22nd Aug 2013
Publication Type: Not specified
PubMed ID: 23865479
Citation: FEBS J. 2013 Sep;280(18):4666-80. doi: 10.1111/febs.12438. Epub 2013 Aug 22.
Scales: Not specified