Experimental analysis of pathway fluxes with varying PGK and FBPAase concentrations in the incubation assay.
Investigation: Central Carbon Metabolism of Sulfolobus solfataricus
Study: Pathway optimisation
Assay type: Extracellular Metabolite Concentration
Technology type: Progressive Curve Experiment
Created: 3rd Feb 2013 at 10:18
Last updated: 26th Oct 2017 at 09:57
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SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".
The goal pursued by SysMO is to record and describe the dynamic molecular processes going on in unicellular microorganisms in a comprehensive way and to present these processes in the form of computerized mathematical models.
Systems biology will raise biomedical and biotechnological research to a new quality level and contribute markedly to progress in understanding. Pooling European research ...
Web page: http://sysmo.net/
An investigation in the CCM of S. solfataricus with a focus on the unique temperature adaptations and regulation; using a combined modelling and experimental approach.
Submitter: Jacky Snoep
Assays: Experimental assays of the temperature dependent degradation of GAP and ..., FBPA/ase, FBPAase model, GAPDH, GAPDH modeling, Gluconeogenic model, Model reconstituted system, Modelling pathway optimisation, Modelling temperature dependent degradation of GAP and DHAP, PGK, PGK and FBPAase titrations, PGK modeling, Reconstituted system reference state, TPI, TPI modeling
Snapshots: Snapshot 1
By varying the input or output flux via PGK and FBPAase manipulation respectively the pathway efficiency can be optimised.
Submitter: Jacky Snoep
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Snapshots: No snapshots
Combined plot fo the model simulations and the experimental data for the PGK and FBPAase titrations.
Increasing the amount of PGK in the incubation assay and measuring the 3PG and F6P fluxes.
Increasing the amount of FBPAase in the reconstituted system and measuring the 3PG and F6P fluxes.
Authors: T. Kouril, D. Esser, J. Kort, H. V. Westerhoff, B. Siebers, J. L. Snoep
Date Published: 22nd Aug 2013
Publication Type: Not specified
PubMed ID: 23865479
Citation: FEBS J. 2013 Sep;280(18):4666-80. doi: 10.1111/febs.12438. Epub 2013 Aug 22.