Glycerol Synthesis in Saccharomyces cerevisiae
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Glycerol, a major by-product of ethanol fermentation by Saccharomyces cerevisiae, is of significant importance to the wine, beer, and ethanol production industries. This investigation aims to gain a clearer understanding of and to quantify the extent to which parameters of the pathway affect glycerol flux in S. cerevisiae.
This investigation represents the data and models submitted to JWS online by Cronwright et al (http://jjj.biochem.sun.ac.za/cgi-bin/getMoreInfo.py?modeldir=cronwright) and described in the publication:
Cronwright GR, Rohwer JM, Prior BA. Metabolic control analysis of glycerol synthesis in Saccharomyces cerevisiae. Appl Environ Microbiol 2002 Sep;68(9):4448-56.
SEEK ID: https://demo.fairdomhub.org/investigations/4
Projects: SysMO DB
Investigation position:
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Created: 19th Aug 2011 at 09:32
Last updated: 4th Nov 2011 at 10:06
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I am a Research Fellow at the University of Manchester, working in Bioinformatics and Computer Science. I am also a guest researcher at the Vrije Universiteit.My research interests include scientific workflows, semantic discovery, and applying ontology technologies to biological data
SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".
The goal pursued by SysMO is to record and describe the dynamic molecular processes going on in unicellular microorganisms in a comprehensive way and to present these processes in the form of computerized mathematical models.
Systems biology will raise biomedical and biotechnological research to a new quality level and contribute markedly to progress in understanding. Pooling European research ...
Projects: BaCell-SysMO, COSMIC, SUMO, KOSMOBAC, SysMO-LAB, PSYSMO, SCaRAB, MOSES, TRANSLUCENT, STREAM, SulfoSys, SysMO DB
Web page: http://sysmo.net/
The main objectives of SysMO-DB are to: facilitate the web-based exchange of data between research groups within- and inter- consortia, and to provide an integrated platform for the dissemination of the results of the SysMO projects to the scientific community. We aim to devise a progressive and scalable solution to the data management needs of the SysMO initiative, that:
- facilitates and maximises the potential for data exchange between SysMO research groups;
- maximises the ‘shelf life’ and ...
Programme: SysMO
Public web page: http://www.sysmo-db.org/
This is a study of the glycerol synthesis pathway, using model predictions and experimental measurements
Submitter: Katy Wolstencroft
Investigation: Glycerol Synthesis in Saccharomyces cerevisiae
Assays: Extracellular Glycerol, Glycerol Synthesis Model construction, Intracellular Metabolites
A kinetic model of the glycerol synthesis pathway has been constructed. Kinetic parameters were collected from published values. Maximal enzyme activities and intracellular effector concentrations were determined experimentally.
Submitter: Katy Wolstencroft
Biological problem addressed: Metabolism
Investigation: Glycerol Synthesis in Saccharomyces cerevisiae
Study: Glycerol Synthesis Pathway
An assay to measure exttracellular glycerol using HPLC
Submitter: Katy Wolstencroft
Assay type: Metabolite Profiling
Technology type: HPLC
Investigation: Glycerol Synthesis in Saccharomyces cerevisiae
Study: Glycerol Synthesis Pathway
Intracellular metabolite concentrations were determined enzymatically by measuring the oxidation or reduction of NADH or NAD+, respectively, at 340 nm in a Beckman Coulter DU640 spectrophotometer
Submitter: Katy Wolstencroft
Assay type: Intracellular Metabolite Concentration
Technology type: Enzymatic Activity Measurements
Investigation: Glycerol Synthesis in Saccharomyces cerevisiae
Study: Glycerol Synthesis Pathway
Intracellular metabolite measurements used for validation of the gycerol synthesis pathway model
Creator: Jacky Snoep
Submitter: Katy Wolstencroft
MCA of Glycerol Synthesis in Saccharomyces cerevisiae
Creators: Jacky Snoep, Garth R. Cronwright, Johann M. Rohwer, and Bernard A. Prior
Submitter: Katy Wolstencroft
Model type: Ordinary differential equations (ODE)
Model format: SBML
Environment: Not specified
Abstract (Expand)
Authors: Garth R Cronwright, Johann M Rohwer, Bernard A Prior
Date Published: 30th Aug 2002
Publication Type: Not specified
