ExploreIncreasing the amount of PGK in the incubation assay and measuring the 3PG and F6P fluxes.
SEEK ID: https://demo.fairdomhub.org/data_files/53?version=1
Filename:
PGK_titration.xlsx
Format: Spreadsheet
Size: 35.6 KB
Contributor and Creators
Views: 1348 Downloads: 113
Created: 3rd Feb 2013 at 11:03
Last updated: 28th May 2015 at 10:50
Last used: 19th Aug 2018 at 08:38
AttributionsNone
ScalesRelated items
Projects: PSYSMO, MOSES, SysMO DB, SysMO-LAB, SulfoSys
Institutions: Manchester Centre for Integrative Systems Biology, University of Manchester, University of Stellenbosch
Silicon cell model for the central carbohydrate metabolism of the archaeon Sulfolobus solfataricus under temperature variation
Programme: SysMO
Public web page: http://sulfosys.com/
An investigation in the CCM of S. solfataricus with a focus on the unique temperature adaptations and regulation; using a combined modelling and experimental approach.
Snapshots: Snapshot 1
Scales: Not specified
By varying the input or output flux via PGK and FBPAase manipulation respectively the pathway efficiency can be optimised.
Person responsible: Jacky Snoep
Snapshots: No snapshots
Scales: Not specified
Varying the relative amounts of PGK and FBPAase and analyse the 3PG uptake and F6P production rates.
Scales: Not specified
Experimental analysis of pathway fluxes with varying PGK and FBPAase concentrations in the incubation assay.
Contributor: Jacky Snoep
Assay type: Extracellular Metabolite Concentration
Technology type: Progressive Curve Experiment
Snapshots: No snapshots
Scales: Not specified
