ExploreThe purified enzymes, PGK, GAPDH, TPI and FBPAase were incubated at 70 C en conversion of 3PG to F6P was followed
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ValidationReference.xlsx
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Views: 3735 Downloads: 225
Created: 2nd Feb 2013 at 17:24
Last updated: 28th May 2015 at 10:50
Last used: 7th Jul 2020 at 10:36
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ScalesRelated items
Silicon cell model for the central carbohydrate metabolism of the archaeon Sulfolobus solfataricus under temperature variation
Programme: SysMO
Public web page: http://sulfosys.com/
An investigation in the CCM of S. solfataricus with a focus on the unique temperature adaptations and regulation; using a combined modelling and experimental approach.
Snapshots: Snapshot 1
Scales: Not specified
Mathematical model of a subset of reactions comprising the three most temperature sensitive intermediates of the gluconeogenic pathway in S. solfataricus
Person responsible: Jacky Snoep
Snapshots: No snapshots
Scales: Not specified
Person responsible: Jacky Snoep
Snapshots: No snapshots
Scales: Not specified
Mathematical model for the analysis of carbon loss of BPG, GAP and DHAP in reconstituted system of S. solfataricus at 70C.
Scales: Not specified
Mathematical model for the reconstituted system with PGK, GAPDH, TPI and FBPAase.
Scales: Not specified
The four purified enzymes were incubated in assay buffer and consumption of 3PG and production of F6P were measured in time, together with GAP and DHAP concentrations.
Contributor: Jacky Snoep
Assay type: Experimental Assay Type
Technology type: Progressive Curve Experiment
Snapshots: No snapshots
Scales: Not specified
