Assays

What is an Assay?

30 Assays visible to you, out of a total of 42

Glycolysis in Saccharomyces cerevisiae

SysMO DB

Glycolysis in Saccharomyces cerevisiae

Submitter: Guest

Biological problem addressed: Metabolism

Investigation: Growth control of the eukaryote cell: a systems...

Study: Can yeast glycolysis be understood in terms of ...

Organisms: Saccharomyces cerevisiae (chemostat)

Models: Teusink and 1 hidden item

SOPs: No SOPs

Data files: No Data files

Snapshots: No snapshots

Created: 5th Jul 2010 at 14:30, Last updated: 26th Oct 2017 at 09:57

NAD+ and NADH Kinetics, using 13C NMR

SysMO DB

No description specified

Submitter: Katy Wolstencroft

Assay type: Glucose Pulse

Technology type: NMR

Investigation: Glycolitic Flux in Lactococcus lactis

Study: The effects of Redox Charge on Glycolytic Flux

Organisms: Escherichia coli

SOPs: No SOPs

Data files: No Data files

Snapshots: No snapshots

Created: 8th Aug 2011 at 13:52, Last updated: 26th Oct 2017 at 09:57

Time dependent responses of glycolytic intermediates in a detailed glycolytic model of Lactococcus lactis during glucose run-out experiments

SysMO DB

Detailed glycolytic model in Lactococcus lactis

Submitter: Katy Wolstencroft

Biological problem addressed: Metabolic Network

Investigation: Glycolitic Flux in Lactococcus lactis

Study: The effects of Redox Charge on Glycolytic Flux

Organisms: Lactococcus lactis (chemostat)

Models: Lactococcus lactis glycolysis

SOPs: No SOPs

Data files: Neves_timeSeries

Snapshots: No snapshots

Created: 8th Aug 2011 at 14:03, Last updated: 26th Oct 2017 at 09:57

Glycerol Synthesis Model construction

SysMO DB

A kinetic model of the glycerol synthesis pathway has been constructed. Kinetic parameters were collected from published values. Maximal enzyme activities and intracellular effector concentrations were determined experimentally.

Submitter: Katy Wolstencroft

Biological problem addressed: Metabolism

Investigation: Glycerol Synthesis in Saccharomyces cerevisiae

Study: Glycerol Synthesis Pathway

Organisms: Saccharomyces cerevisiae

Models: Metabolic Control Analysis of Glycerol Synthesi...

SOPs: No SOPs

Data files: Cronwright Parameter Data

Snapshots: No snapshots

Created: 19th Aug 2011 at 09:23, Last updated: 26th Oct 2017 at 09:57

Extracellular Glycerol

SysMO DB

An assay to measure exttracellular glycerol using HPLC

Submitter: Katy Wolstencroft

Assay type: Metabolite Profiling

Technology type: HPLC

Investigation: Glycerol Synthesis in Saccharomyces cerevisiae

Study: Glycerol Synthesis Pathway

Organisms: Saccharomyces cerevisiae

SOPs: No SOPs

Data files: 1 hidden item

Snapshots: No snapshots

Created: 19th Aug 2011 at 10:02, Last updated: 26th Oct 2017 at 09:57

Intracellular Metabolites

SysMO DB

Intracellular metabolite concentrations were determined enzymatically by measuring the oxidation or reduction of NADH or NAD+, respectively, at 340 nm in a Beckman Coulter DU640 spectrophotometer

Submitter: Katy Wolstencroft

Assay type: Intracellular Metabolite Concentration

Technology type: Enzymatic Activity Measurements

Investigation: Glycerol Synthesis in Saccharomyces cerevisiae

Study: Glycerol Synthesis Pathway

Organisms: Saccharomyces cerevisiae

SOPs: No SOPs

Data files: Cronwright Parameter Data

Snapshots: No snapshots

Created: 19th Aug 2011 at 11:28, Last updated: 26th Oct 2017 at 09:57

PGK