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Glycolysis in Saccharomyces cerevisiae

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Detailed glycolytic model in Lactococcus lactis

A kinetic model of the glycerol synthesis pathway has been constructed. Kinetic parameters were collected from published values. Maximal enzyme activities and intracellular effector concentrations were determined experimentally.

Submitter: Katy Wolstencroft

Biological problem addressed: Metabolism

Investigation: Glycerol Synthesis in Saccharomyces cerevisiae

Study: Glycerol Synthesis Pathway

An assay to measure exttracellular glycerol using HPLC

Intracellular metabolite concentrations were determined enzymatically by measuring the oxidation or reduction of NADH or NAD+, respectively, at 340 nm in a Beckman Coulter DU640 spectrophotometer

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