1. Home
  2. Data files Index
  3. Model simulation and Exp data for GAP T deg

Model simulation and Exp data for GAP T deg
Version 1

Download View content

A time simulation of the mathematical model is shown together with the experimental data to which the model was fitted.

SEEK ID: https://demo.fairdomhub.org/data_files/44?version=1

Filename: TdegGAP.jpg  Download

Format: JPEG image

Size: 9.85 KB

Help
help Creators and Submitter
Creator
Submitter
License
No license - no permission to use unless the owner grants a licence
Activity

Views: 5946   Downloads: 287

Created: 29th Jan 2013 at 22:01

Last updated: 28th May 2015 at 08:50

Last used: 25th Jan 2021 at 02:00

help Attributions

None

help Scales
Not Specified

Related items

SulfoSys

Silicon cell model for the central carbohydrate metabolism of the archaeon Sulfolobus solfataricus under temperature variation

Programme: SysMO

Public web page: http://sulfosys.com/

Central Carbon Metabolism of Sulfolobus solfataricus
SulfoSys

An investigation in the CCM of S. solfataricus with a focus on the unique temperature adaptations and regulation; using a combined modelling and experimental approach.

Snapshots: Snapshot 1

Model gluconeogenesis
SulfoSys

Mathematical model of a subset of reactions comprising the three most temperature sensitive intermediates of the gluconeogenic pathway in S. solfataricus

Person responsible: Jacky Snoep

Snapshots: No snapshots

Modelling temperature dependent degradation of GAP and DHAP
SulfoSys

The temperature dependent degradation of DHAP and GAP is modelled as an exponential decay process using experimental data for the disintegration at 70C.

Submitter: Jacky Snoep

Biological problem addressed: Metabolism

Snapshots: No snapshots

Intermediate instability at high temperature leads to low pathway efficiency for an in vitro reconstituted system of gluconeogenesis in Sulfolobus solfataricus
SulfoSys

Abstract (Expand)

Four enzymes of the gluconeogenic pathway in Sulfolobus solfataricus were purified and kinetically characterized. The enzymes were reconstituted in vitro to quantify the contribution of temperature … instability of the pathway intermediates to carbon loss from the system. The reconstituted system, consisting of phosphoglycerate kinase, glyceraldehyde 3-phosphate dehydrogenase, triose phosphate isomerase and the fructose 1,6-bisphosphate aldolase/phosphatase, maintained a constant consumption rate of 3-phosphoglycerate and production of fructose 6-phosphate over a 1-h period. Cofactors ATP and NADPH were regenerated via pyruvate kinase and glucose dehydrogenase. A mathematical model was constructed on the basis of the kinetics of the purified enzymes and the measured half-life times of the pathway intermediates. The model quantitatively predicted the system fluxes and metabolite concentrations. Relative enzyme concentrations were chosen such that half the carbon in the system was lost due to degradation of the thermolabile intermediates dihydroxyacetone phosphate, glyceraldehyde 3-phosphate and 1,3-bisphosphoglycerate, indicating that intermediate instability at high temperature can significantly affect pathway efficiency.

Authors: T. Kouril, D. Esser, J. Kort, H. V. Westerhoff, B. Siebers, J. L. Snoep

Date Published: 22nd Aug 2013

Publication Type: Not specified

Powered by
 (v.1.10.3)
FAIRDOM DEMO | Funding and Programmes | Credits | Imprint
Copyright © 2008 - 2020 The University of Manchester and HITS gGmbH