Assays

What is an Assay?

15 Assays visible to you, out of a total of 15

SulfoSys

Mathematical model for PGK kinetics, saturation with ADP, ATP, 3PG and BPG.

Submitter: Jacky Snoep

Biological problem addressed: Model Analysis Type

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model gluconeogenesis

Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)

Models: 1 hidden item

SOPs: No SOPs

Data files: Model simulation and experimental data for ADP,..., PGK kinetics 3PG, PGK kinetics ADP, PGK kinetics ATP, PGK kinetics BPG

Snapshots: No snapshots

Created: 1st Feb 2013 at 22:44, Last updated: 26th Oct 2017 at 09:57

SulfoSys

Mathematical model for GAPDH kinetics, saturation of GAP, BPG, NADP, NADPH and Pi.

Submitter: Jacky Snoep

Biological problem addressed: Model Analysis Type

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model gluconeogenesis

Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)

Models: GAPDH kinetic model

SOPs: No SOPs

Data files: GAPDH kinetics 3PG, GAPDH kinetics GAP, GAPDH kinetics NADP, GAPDH kinetics NADPH, GAPDH kinetics Pi and 1 hidden item

Snapshots: No snapshots

Created: 1st Feb 2013 at 23:11, Last updated: 26th Oct 2017 at 09:57

SulfoSys

Mathematical model for TPI kinetics, saturation of GAP and DHAP.

Submitter: Jacky Snoep

Biological problem addressed: Model Analysis Type

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model gluconeogenesis

Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)

Models: TIM kinetic model

SOPs: No SOPs

Data files: Model simulation and exp data for TIM kinetics,..., TPI kinetics DHAP, TPI kinetics GAP, TPI kinetics PEP inhibition GAP, TPI kinetics PEP inhibition of DHAP

Snapshots: No snapshots

Created: 1st Feb 2013 at 23:26, Last updated: 26th Oct 2017 at 09:57

Gluconeogenic model

SulfoSys

Mathematical model for the analysis of carbon loss of BPG, GAP and DHAP in reconstituted system of S. solfataricus at 70C.

Submitter: Jacky Snoep

Biological problem addressed: Model Analysis Type

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model gluconeogenesis

Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)

Models: Mathematical model for the combined four enzyme...

SOPs: No SOPs

Data files: Metabolite concentrations during reconstituted ...

Snapshots: No snapshots

Created: 2nd Feb 2013 at 13:21, Last updated: 26th Oct 2017 at 09:57

Reconstituted system reference state

SulfoSys

The four purified enzymes were incubated in assay buffer and consumption of 3PG and production of F6P were measured in time, together with GAP and DHAP concentrations.

Submitter: Jacky Snoep

Assay type: Experimental Assay Type

Technology type: Progressive Curve Experiment

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Carbon loss at high T

Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)

SOPs: No SOPs

Data files: Metabolite concentrations during reconstituted ..., Model simulation and Exp data for reconstituted...

Snapshots: No snapshots

Created: 2nd Feb 2013 at 16:06, Last updated: 26th Oct 2017 at 09:57

Model reconstituted system

SulfoSys

Mathematical model for the reconstituted system with PGK, GAPDH, TPI and FBPAase.

Submitter: Jacky Snoep

Biological problem addressed: Model Analysis Type

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Carbon loss at high T

Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)

Models: Mathematical model for the combined four enzyme...

SOPs: No SOPs

Data files: Metabolite concentrations during reconstituted ..., Model simulation and Exp data for reconstituted...

Snapshots: No snapshots

Created: 2nd Feb 2013 at 16:32, Last updated: 26th Oct 2017 at 09:57

Modelling pathway optimisation

SulfoSys

Varying the relative amounts of PGK and FBPAase and analyse the 3PG uptake and F6P production rates.

Submitter: Jacky Snoep

Biological problem addressed: Model Analysis Type

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Pathway optimisation

Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)

Models: Mathematical model for the combined four enzyme...

SOPs: No SOPs

Data files: FBPAase titration of reconstituted system, Model simulation and experimental data for PGK ..., PGK titration of reconstituted system, Pathway efficiency for 3PG to F6P conversion as...

Snapshots: No snapshots

Created: 3rd Feb 2013 at 09:56, Last updated: 26th Oct 2017 at 09:57

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