Kinetic characterisation of Phosphoglycerokinase
Submitter: Jacky Snoep
Assay type: Experimental Assay Type
Technology type: Initial Rate Experiment
Snapshots: No snapshots
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Model gluconeogenesis
Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)
SOPs: Expression of S. solfataricus enzymes in E. col..., Preparation of cell free extract of recombinant..., Protein purification from E. coli extracts.
Data files: Model simulation and experimental data for ADP,..., PGK kinetics 3PG, PGK kinetics ADP, PGK kinetics ATP, PGK kinetics BPG
Data files: GAPDH kinetics 3PG, GAPDH kinetics GAP, GAPDH kinetics NADP, GAPDH kinetics NADPH, GAPDH kinetics Pi
Data files: Model simulation and exp data for TIM kinetics,..., TPI kinetics DHAP, TPI kinetics GAP, TPI kinetics PEP inhibition GAP, TPI kinetics PEP inhibition of DHAP
Kinetic characterization of the FBPA/ase with respect to GAP en DHAP saturation.
Data files: FBPA/ase kinetics DHAP, FBPA/ase kinetics GAP, Model simulation and experimental data for DHAP...
GAP and DHAP concentrations were measured over time at 70C.
Technology type: Progressive Curve Experiment
Organisms: No organisms
SOPs: No SOPs
Data files: T degradation DHAP, T degradation GAP
The temperature dependent degradation of DHAP and GAP is modelled as an exponential decay process using experimental data for the disintegration at 70C.
Biological problem addressed: Metabolism
Models: DHAP T degradation, GAP T degradation
Data files: Model simulation and Exp data for DHAP T deg, Model simulation and Exp data for GAP T deg, T degradation DHAP, T degradation GAP
Mathematical model for FBPAase kinetics, saturation of GAP and DHAP
Biological problem addressed: Model Analysis Type
Models: FBPAase kinetic model