Technology type 'Initial Rate Experiment'

Enzymatic Activity Measurements
Current technology type

Initial Rate Experiment
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Related assays

4 Assays visible to you, out of a total of 4

PGK

SulfoSys

Kinetic characterisation of Phosphoglycerokinase

Submitter: Jacky Snoep

Assay type: Experimental Assay Type

Technology type: Initial Rate Experiment

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model gluconeogenesis

Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)

SOPs: Expression of S. solfataricus enzymes in E. col..., Preparation of cell free extract of recombinant..., Protein purification from E. coli extracts.

Data files: Model simulation and experimental data for ADP,..., PGK kinetics 3PG, PGK kinetics ADP, PGK kinetics ATP, PGK kinetics BPG

Snapshots: No snapshots

Created: 29th Jan 2013 at 14:18, Last updated: 26th Oct 2017 at 09:57

GAPDH

SulfoSys

No description specified

Submitter: Jacky Snoep

Assay type: Experimental Assay Type

Technology type: Initial Rate Experiment

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model gluconeogenesis

Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)

SOPs: Expression of S. solfataricus enzymes in E. col..., Preparation of cell free extract of recombinant..., Protein purification from E. coli extracts.

Data files: GAPDH kinetics 3PG, GAPDH kinetics GAP, GAPDH kinetics NADP, GAPDH kinetics NADPH, GAPDH kinetics Pi

Snapshots: No snapshots

Created: 29th Jan 2013 at 20:37, Last updated: 26th Oct 2017 at 09:57

TPI

SulfoSys

No description specified

Submitter: Jacky Snoep

Assay type: Experimental Assay Type

Technology type: Initial Rate Experiment

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model gluconeogenesis

Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)

SOPs: Expression of S. solfataricus enzymes in E. col..., Preparation of cell free extract of recombinant..., Protein purification from E. coli extracts.

Data files: Model simulation and exp data for TIM kinetics,..., TPI kinetics DHAP, TPI kinetics GAP, TPI kinetics PEP inhibition GAP, TPI kinetics PEP inhibition of DHAP

Snapshots: No snapshots

Created: 29th Jan 2013 at 20:45, Last updated: 26th Oct 2017 at 09:57

FBPA/ase

SulfoSys

Kinetic characterization of the FBPA/ase with respect to GAP en DHAP saturation.

Submitter: Jacky Snoep

Assay type: Experimental Assay Type

Technology type: Initial Rate Experiment

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model gluconeogenesis

Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)

SOPs: Expression of S. solfataricus enzymes in E. col..., Preparation of cell free extract of recombinant..., Protein purification from E. coli extracts.

Data files: FBPA/ase kinetics DHAP, FBPA/ase kinetics GAP, Model simulation and experimental data for DHAP...

Snapshots: No snapshots

Created: 29th Jan 2013 at 20:51, Last updated: 26th Oct 2017 at 09:57