Assays

Mathematical model for FBPAase kinetics, saturation of GAP and DHAP

Mathematical model for GAPDH kinetics, saturation of GAP, BPG, NADP, NADPH and Pi.

Mathematical model for the analysis of carbon loss of BPG, GAP and DHAP in reconstituted system of S. solfataricus at 70C.

Mathematical model for the reconstituted system with PGK, GAPDH, TPI and FBPAase.

Varying the relative amounts of PGK and FBPAase and analyse the 3PG uptake and F6P production rates.

Experimental analysis of pathway fluxes with varying PGK and FBPAase concentrations in the incubation assay.

Mathematical model for PGK kinetics, saturation with ADP, ATP, 3PG and BPG.

The four purified enzymes were incubated in assay buffer and consumption of 3PG and production of F6P were measured in time, together with GAP and DHAP concentrations.

Mathematical model for TPI kinetics, saturation of GAP and DHAP.

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