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| Metadata | Values (examples) | ||||||||
| Asset Title | PGK_ADP | ||||||||
| Uploader | Jacky Snoep | ||||||||
| Uploader SEEK ID | 49 | ||||||||
| Project | SulfoSys | ||||||||
| ASSAY | |||||||||
| Assay SEEK ID | 16 | ||||||||
| Assay Title | PGK | ||||||||
| Assay_type | enzymaticAssay | ||||||||
| Technology_type | initial rate experiment | ||||||||
| Description | Kinetic characterisation of Phosphoglycerokinase. Measures enzyme reaction rate with increasing concentration of ADP and 5mM 1.3BPG | ||||||||
| Experimentalist | Theresa Kouril? | ||||||||
| Date | |||||||||
| SOP | 18 | ||||||||
| Publication (optional) | |||||||||
| Experimental_conditions | |||||||||
| Item | concentration | ||||||||
| Compound (if concentration) | 1.3BPG | ||||||||
| Unit | mM | ||||||||
| Start_value (optional) | 5 | ||||||||
| End_value (optional) | 5 | ||||||||
| Comments | |||||||||
| Culture growth | CultureGrowth | ||||||||
| FACTORS_STUDIED | |||||||||
| Item | concentration | ||||||||
| Compound (if concentration) | ADP | ||||||||
| Unit | mM | ||||||||
| Start_value (optional) | 0 | ||||||||
| End_value (optional) | 5 | ||||||||
| SD (optional) | |||||||||
| Comments | |||||||||
| ENZYME IDENTITY | |||||||||
| Enzyme Name (IUBMB) | Phosphoglycerate Kinase | ||||||||
| EC Number | 2.7.2.3 | ||||||||
| Sequence Accession Number (uniprot) | P50317 | ||||||||
| Reaction | |||||||||
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| Sample Name | Treatments (Factors Studied) | Culture_start_date | Date_at_sampling | Age_at_sampling_hours | |||||||||
| Organism | NCBI_ID | Strain | Genotype | Phenotype | organism/cell part | Identifier | concentration | date | date | age | Description | ||
| PGK_ADP_0 | Sulfolobus solfataricus | 2287 | P2 | wild-type | wild-type | purified protein | P50317 | 0 | |||||
| PGK_ADP_1 | Sulfolobus solfataricus | 2287 | P2 | wild-type | wild-type | purified protein | P50317 | 0.005 | |||||
| PGK_ADP_2 | Sulfolobus solfataricus | 2287 | P2 | wild-type | wild-type | purified protein | P50317 | 0.01 | |||||
| PGK_ADP_3 | Sulfolobus solfataricus | 2287 | P2 | wild-type | wild-type | purified protein | P50317 | 0.02 | |||||
| PGK_ADP_4 | Sulfolobus solfataricus | 2287 | P2 | wild-type | wild-type | purified protein | P50317 | 0.05 | |||||
| PGK_ADP_5 | Sulfolobus solfataricus | 2287 | P2 | wild-type | wild-type | purified protein | P50317 | 0.1 | |||||
| PGK_ADP_6 | Sulfolobus solfataricus | 2287 | P2 | wild-type | wild-type | purified protein | P50317 | 0.2 | |||||
| PGK_ADP_7 | Sulfolobus solfataricus | 2287 | P2 | wild-type | wild-type | purified protein | P50317 | 0.5 | |||||
| PGK_ADP_8 | Sulfolobus solfataricus | 2287 | P2 | wild-type | wild-type | purified protein | P50317 | 1 | |||||
| PGK_ADP_9 | Sulfolobus solfataricus | 2287 | P2 | wild-type | wild-type | purified protein | P50317 | 2 | |||||
| PGK_ADP_10 | Sulfolobus solfataricus | 2287 | P2 | wild-type | wild-type | purified protein | P50317 | 3 | |||||
| PGK_ADP_11 | Sulfolobus solfataricus | 2287 | P2 | wild-type | wild-type | purified protein | P50317 | 5 | |||||
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| Samples | Substrate Concentration | rate | SD | ||||||||||||||||||||||||||||||
| ADP | |||||||||||||||||||||||||||||||||
| mM | U/mg | U/mg | |||||||||||||||||||||||||||||||
| PGK_ADP_0 | 0 | 0 | 0 | ||||||||||||||||||||||||||||||
| PGK_ADP_1 | 0.005 | 4.758343062852696 | 0.9076657572957979 | ||||||||||||||||||||||||||||||
| PGK_ADP_2 | 0.01 | 6.069547820587665 | 0.34963394605305737 | ||||||||||||||||||||||||||||||
| PGK_ADP_3 | 0.02 | 7.85202617240708 | 0.13312931662392874 | ||||||||||||||||||||||||||||||
| PGK_ADP_4 | 0.05 | 11.709628575598366 | 1.3596513692352699 | ||||||||||||||||||||||||||||||
| PGK_ADP_5 | 0.1 | 13.047437487838103 | 0.18976344699871273 | ||||||||||||||||||||||||||||||
| PGK_ADP_6 | 0.2 | 14.643686758124147 | 1.219137746108125 | ||||||||||||||||||||||||||||||
| PGK_ADP_7 | 0.5 | 14.202817912045143 | 0.8539418546800315 | ||||||||||||||||||||||||||||||
| PGK_ADP_8 | 1 | 12.944821463319713 | 0.2982679047210616 | ||||||||||||||||||||||||||||||
| PGK_ADP_9 | 2 | 10.546646964389957 | 0.9347605879775849 | ||||||||||||||||||||||||||||||
| PGK_ADP_10 | 3 | 9.182233897645455 | 0.6670772437021008 | ||||||||||||||||||||||||||||||
| PGK_ADP_11 | 5 | 9.110022621132515 | 1.6781288093496816 | ||||||||||||||||||||||||||||||
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| This is an optional sheet to show any derived results that may be appropriate | |||||||||
