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| Metadata | Values (examples) | ||||||||
| Asset Title | PGK_3PG | ||||||||
| Uploader | Jacky Snoep | ||||||||
| Uploader SEEK ID | 49 | ||||||||
| Project | SulfoSys | ||||||||
| ASSAY | |||||||||
| Assay SEEK ID | 16 | ||||||||
| Assay Title | PGK | ||||||||
| Assay_type | enzymaticAssay | ||||||||
| Technology_type | initial rate experiment | ||||||||
| Description | Kinetic characterisation of Phosphoglycerokinase. Measures enzyme reaction rate with increasing concentration of 3PG and 10mM ATP | ||||||||
| Experimentalist | Theresa Kouril? | ||||||||
| Date | |||||||||
| SOP | 18 | ||||||||
| Publication (optional) | |||||||||
| Experimental_conditions | |||||||||
| Item | concentration | ||||||||
| Compound (if concentration) | ATP | ||||||||
| Unit | mM | ||||||||
| Start_value (optional) | 10 | ||||||||
| End_value (optional) | 10 | ||||||||
| Comments | |||||||||
| Culture growth | CultureGrowth | ||||||||
| FACTORS_STUDIED | |||||||||
| Item | concentration | ||||||||
| Compound (if concentration) | 3PG | ||||||||
| Unit | mM | ||||||||
| Start_value (optional) | 0 | ||||||||
| End_value (optional) | 15 | ||||||||
| SD (optional) | |||||||||
| Comments | |||||||||
| ENZYME IDENTITY | |||||||||
| Enzyme Name (IUBMB) | Phosphoglycerate Kinase | ||||||||
| EC Number | 2.7.2.3 | ||||||||
| Sequence Accession Number (uniprot) | P50317 | ||||||||
| Reaction | |||||||||
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| Sample Name | Treatments (Factors Studied) | Culture_start_date | Date_at_sampling | Age_at_sampling_hours | |||||||||
| Organism | NCBI_ID | Strain | Genotype | Phenotype | organism/cell part | Identifier | concentration | date | date | age | Description | ||
| PGK_3PG_0 | Sulfolobus solfataricus | 2287 | P2 | wild-type | wild-type | purified protein | P50317 | 0 | |||||
| PGK_3PG_1 | Sulfolobus solfataricus | 2287 | P2 | wild-type | wild-type | purified protein | P50317 | 0.06 | |||||
| PGK_3PG_2 | Sulfolobus solfataricus | 2287 | P2 | wild-type | wild-type | purified protein | P50317 | 0.1 | |||||
| PGK_3PG_3 | Sulfolobus solfataricus | 2287 | P2 | wild-type | wild-type | purified protein | P50317 | 0.2 | |||||
| PGK_3PG_4 | Sulfolobus solfataricus | 2287 | P2 | wild-type | wild-type | purified protein | P50317 | 0.4 | |||||
| PGK_3PG_5 | Sulfolobus solfataricus | 2287 | P2 | wild-type | wild-type | purified protein | P50317 | 1 | |||||
| PGK_3PG_6 | Sulfolobus solfataricus | 2287 | P2 | wild-type | wild-type | purified protein | P50317 | 2 | |||||
| PGK_3PG_7 | Sulfolobus solfataricus | 2287 | P2 | wild-type | wild-type | purified protein | P50317 | 5 | |||||
| PGK_3PG_8 | Sulfolobus solfataricus | 2287 | P2 | wild-type | wild-type | purified protein | P50317 | 10 | |||||
| PGK_3PG_9 | Sulfolobus solfataricus | 2287 | P2 | wild-type | wild-type | purified protein | P50317 | 15 | |||||
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| Samples | Substrate Concentration | rate | SD | ||||||||||||||||||||||||||||||
| 3PG | |||||||||||||||||||||||||||||||||
| mM | U/mg | U/mg | |||||||||||||||||||||||||||||||
| PGK_3PG_0 | 0 | 1.6817626240513717 | 0.1374434865190047 | ||||||||||||||||||||||||||||||
| PGK_3PG_1 | 0.06 | 3.8699191413879337 | 0.04471986492631012 | ||||||||||||||||||||||||||||||
| PGK_3PG_2 | 0.1 | 4.863967890559123 | 0.2449551492414605 | ||||||||||||||||||||||||||||||
| PGK_3PG_3 | 0.2 | 6.104820612737502 | 0.20284888857605468 | ||||||||||||||||||||||||||||||
| PGK_3PG_4 | 0.4 | 7.424089901434563 | 0.25571313236354 | ||||||||||||||||||||||||||||||
| PGK_3PG_5 | 1 | 8.51256697678008 | 0.3017459955371954 | ||||||||||||||||||||||||||||||
| PGK_3PG_6 | 2 | 9.39687945373253 | 0.26831066546844856 | ||||||||||||||||||||||||||||||
| PGK_3PG_7 | 5 | 10.183743071095757 | 0.0986035946098972 | ||||||||||||||||||||||||||||||
| PGK_3PG_8 | 10 | 10.565591280759094 | 0.45947336675721195 | ||||||||||||||||||||||||||||||
| PGK_3PG_9 | 15 | 10.509902028769949 | 0.2535200095729727 | ||||||||||||||||||||||||||||||
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| This is an optional sheet to show any derived results that may be appropriate | |||||||||
